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primescript tm ii 1 st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript tm ii 1 st strand cdna synthesis kit
    Primescript Tm Ii 1 St Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+tm+ii+1+st+strand+cdna+synthesis+kit/PrimeScript+Double+Strand+cDNA+Synthesis+Kit/pmc12470604-62-13-22
    Average 96 stars, based on 1667 article reviews
    primescript tm ii 1 st strand cdna synthesis kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: miR-9875 functions in antiviral immunity by targeting PDCD6 in mud crab ( Scylla paramamosain )
    Article Snippet: .. Total RNA was extracted using MagMAX TM mirVana TM Total RNA Isolation Kit (Thermo Fisher Scientific, USA), followed by first-strand cDNA synthesis via PrimeScript TM II 1 st Strand cDNA Synthesis Kit (Takara, Japan) using (5ʹ-GTCGTATCCAGTGCAGGGTCCGAGGTCACTGGATACGACCTCCTCCC-3ʹ). .. The qPCR was carried out using the Premix Ex Taq (TaKaRa, Japan) to quantify the expression level of miR-9875.

    Article Title: Long non-coding RNA MEG3 acts as a suppressor in breast cancer by regulating miR-330/CNN1
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, USA) and reversed to cDNA with the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TAKARA, Japan). .. The ABI Viia 7 Real-Time PCR system (Applied Biosystems, Foster City, CA) was used to perform qPCR with primer sequences listed in .

    Article Title: Low glucose and serum levels cause an increased inflammatory factor in 3T3-L1 cell through Akt, MAPKs and NF-кB activation
    Article Snippet: The gels stained with 0.1% amido black 10B (Wako Pure Chemical Industries, Osaka, Japan). .. Total RNA was isolated using Sepasol-RNA II Super (Nacalai Tesque, Kyoto, Japan) and TURBO DNA-free TM Kit (Thermo Fisher Scientific, MA, USA). cDNA was synthesized using PrimeScript TM II 1 st strand cDNA Synthesis Kit (Takara Bio. ..

    cDNA Synthesis:

    Article Title: miR-9875 functions in antiviral immunity by targeting PDCD6 in mud crab ( Scylla paramamosain )
    Article Snippet: .. Total RNA was extracted using MagMAX TM mirVana TM Total RNA Isolation Kit (Thermo Fisher Scientific, USA), followed by first-strand cDNA synthesis via PrimeScript TM II 1 st Strand cDNA Synthesis Kit (Takara, Japan) using (5ʹ-GTCGTATCCAGTGCAGGGTCCGAGGTCACTGGATACGACCTCCTCCC-3ʹ). .. The qPCR was carried out using the Premix Ex Taq (TaKaRa, Japan) to quantify the expression level of miR-9875.

    Article Title: Replication Factor C Subunit 4 Plays a Role in Human Breast Cancer Cell Progression
    Article Snippet: .. Total RNA was extracted from breast cancer cells using the TRIzol Reagent (Invitrogen) and cDNA was synthesized using the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa Bio Inc., Shiga, Japan) according to the manufacturer’s protocols. qRT-PCR was performed using a CFX96 real-time PCR detection system (Bio-Rad Laboratories Inc., Hercules, CA, USA) with TB Green Premix Ex Taq (TaKaRa Bio Inc.). ..

    Article Title: Long non-coding RNA MEG3 acts as a suppressor in breast cancer by regulating miR-330/CNN1
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, USA) and reversed to cDNA with the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TAKARA, Japan). .. The ABI Viia 7 Real-Time PCR system (Applied Biosystems, Foster City, CA) was used to perform qPCR with primer sequences listed in .

    Article Title: Synergistic regulation of methylation and SP1 on MAGE-D4 transcription in glioma
    Article Snippet: Quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted from glioma tissues and cell lines using a Universal RNA Extract Kit (TaKaRa). .. First-strand cDNA was synthesized from total RNA using PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. qRT-PCR was performed on the StepOneTM Real-Time PCR System (Applied Biosystems) using SYBR Green I (Roche). ..

    Article Title: Proteasome Subunits Regulate Reproduction in Nilaparvata lugens and the Transovarial Transmission of Its Yeast-like Symbionts
    Article Snippet: Total RNA was extracted from adult female N. lugens samples using the MiniBEST Universal RNA Extraction Kit (Takara Bio, Dalian, China). .. The cDNA was obtained by reverse transcription of the synthesized RNA using the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (Takara Bio), with random primer (N7) and Oligo (dT). ..

    Article Title: Identification and characterization of cold-responsive aquaporins from the larvae of a crambid pest Agriphila aeneociliella (Eversmann) (Lepidoptera: Crambidae)
    Article Snippet: The quality of total RNA was then detected using ultraviolet spectrophotometry (NanoDrop One; Thermo Fisher, Waltham, MA, USA) (OD260/OD280 = 1.92, OD260/OD230 = 2.0). .. First strand cDNA was reverse transcribed from RNA using PrimeScript II Rtase with the Oligo (dT) primer and Random 6 primer (PrimeScript TM II 1 st Strand cDNA Synthesis Kit, Takara Bio, Beijing, China). ..

    Article Title: Low glucose and serum levels cause an increased inflammatory factor in 3T3-L1 cell through Akt, MAPKs and NF-кB activation
    Article Snippet: The gels stained with 0.1% amido black 10B (Wako Pure Chemical Industries, Osaka, Japan). .. Total RNA was isolated using Sepasol-RNA II Super (Nacalai Tesque, Kyoto, Japan) and TURBO DNA-free TM Kit (Thermo Fisher Scientific, MA, USA). cDNA was synthesized using PrimeScript TM II 1 st strand cDNA Synthesis Kit (Takara Bio. ..

    Synthesized:

    Article Title: Replication Factor C Subunit 4 Plays a Role in Human Breast Cancer Cell Progression
    Article Snippet: .. Total RNA was extracted from breast cancer cells using the TRIzol Reagent (Invitrogen) and cDNA was synthesized using the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa Bio Inc., Shiga, Japan) according to the manufacturer’s protocols. qRT-PCR was performed using a CFX96 real-time PCR detection system (Bio-Rad Laboratories Inc., Hercules, CA, USA) with TB Green Premix Ex Taq (TaKaRa Bio Inc.). ..

    Article Title: Synergistic regulation of methylation and SP1 on MAGE-D4 transcription in glioma
    Article Snippet: Quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted from glioma tissues and cell lines using a Universal RNA Extract Kit (TaKaRa). .. First-strand cDNA was synthesized from total RNA using PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. qRT-PCR was performed on the StepOneTM Real-Time PCR System (Applied Biosystems) using SYBR Green I (Roche). ..

    Article Title: Proteasome Subunits Regulate Reproduction in Nilaparvata lugens and the Transovarial Transmission of Its Yeast-like Symbionts
    Article Snippet: Total RNA was extracted from adult female N. lugens samples using the MiniBEST Universal RNA Extraction Kit (Takara Bio, Dalian, China). .. The cDNA was obtained by reverse transcription of the synthesized RNA using the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (Takara Bio), with random primer (N7) and Oligo (dT). ..

    Article Title: Low glucose and serum levels cause an increased inflammatory factor in 3T3-L1 cell through Akt, MAPKs and NF-кB activation
    Article Snippet: The gels stained with 0.1% amido black 10B (Wako Pure Chemical Industries, Osaka, Japan). .. Total RNA was isolated using Sepasol-RNA II Super (Nacalai Tesque, Kyoto, Japan) and TURBO DNA-free TM Kit (Thermo Fisher Scientific, MA, USA). cDNA was synthesized using PrimeScript TM II 1 st strand cDNA Synthesis Kit (Takara Bio. ..

    Quantitative RT-PCR:

    Article Title: Replication Factor C Subunit 4 Plays a Role in Human Breast Cancer Cell Progression
    Article Snippet: .. Total RNA was extracted from breast cancer cells using the TRIzol Reagent (Invitrogen) and cDNA was synthesized using the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa Bio Inc., Shiga, Japan) according to the manufacturer’s protocols. qRT-PCR was performed using a CFX96 real-time PCR detection system (Bio-Rad Laboratories Inc., Hercules, CA, USA) with TB Green Premix Ex Taq (TaKaRa Bio Inc.). ..

    Article Title: Synergistic regulation of methylation and SP1 on MAGE-D4 transcription in glioma
    Article Snippet: Quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted from glioma tissues and cell lines using a Universal RNA Extract Kit (TaKaRa). .. First-strand cDNA was synthesized from total RNA using PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. qRT-PCR was performed on the StepOneTM Real-Time PCR System (Applied Biosystems) using SYBR Green I (Roche). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Replication Factor C Subunit 4 Plays a Role in Human Breast Cancer Cell Progression
    Article Snippet: .. Total RNA was extracted from breast cancer cells using the TRIzol Reagent (Invitrogen) and cDNA was synthesized using the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa Bio Inc., Shiga, Japan) according to the manufacturer’s protocols. qRT-PCR was performed using a CFX96 real-time PCR detection system (Bio-Rad Laboratories Inc., Hercules, CA, USA) with TB Green Premix Ex Taq (TaKaRa Bio Inc.). ..

    Article Title: Synergistic regulation of methylation and SP1 on MAGE-D4 transcription in glioma
    Article Snippet: Quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted from glioma tissues and cell lines using a Universal RNA Extract Kit (TaKaRa). .. First-strand cDNA was synthesized from total RNA using PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. qRT-PCR was performed on the StepOneTM Real-Time PCR System (Applied Biosystems) using SYBR Green I (Roche). ..

    SYBR Green Assay:

    Article Title: Synergistic regulation of methylation and SP1 on MAGE-D4 transcription in glioma
    Article Snippet: Quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted from glioma tissues and cell lines using a Universal RNA Extract Kit (TaKaRa). .. First-strand cDNA was synthesized from total RNA using PrimeScript TM II 1 st Strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols. qRT-PCR was performed on the StepOneTM Real-Time PCR System (Applied Biosystems) using SYBR Green I (Roche). ..

    Reverse Transcription:

    Article Title: Proteasome Subunits Regulate Reproduction in Nilaparvata lugens and the Transovarial Transmission of Its Yeast-like Symbionts
    Article Snippet: Total RNA was extracted from adult female N. lugens samples using the MiniBEST Universal RNA Extraction Kit (Takara Bio, Dalian, China). .. The cDNA was obtained by reverse transcription of the synthesized RNA using the PrimeScript TM II 1 st Strand cDNA Synthesis Kit (Takara Bio), with random primer (N7) and Oligo (dT). ..

    Article Title: Identification and characterization of cold-responsive aquaporins from the larvae of a crambid pest Agriphila aeneociliella (Eversmann) (Lepidoptera: Crambidae)
    Article Snippet: The quality of total RNA was then detected using ultraviolet spectrophotometry (NanoDrop One; Thermo Fisher, Waltham, MA, USA) (OD260/OD280 = 1.92, OD260/OD230 = 2.0). .. First strand cDNA was reverse transcribed from RNA using PrimeScript II Rtase with the Oligo (dT) primer and Random 6 primer (PrimeScript TM II 1 st Strand cDNA Synthesis Kit, Takara Bio, Beijing, China). ..



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    TaKaRa primescript tm ii 1 st strand cdna synthesis kit for qpcr
    Expression levels of the screened seven genes after si-MEF2A transfection. (A–H) After BV2 cells transfected with three si-MEF2A for 48 h to knock down the expression of MEF2A, the expression levels of MEF2A, BNIP3, CDK5R1, HERC1, ITPR1, OPTN, UBQLN2, and USP33 were detected using <t>qPCR.</t> ANOVA followed by LSD test was used for comparison among groups. * p < 0.05 compared with the control group.
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    Image Search Results


    Expression levels of the screened seven genes after si-MEF2A transfection. (A–H) After BV2 cells transfected with three si-MEF2A for 48 h to knock down the expression of MEF2A, the expression levels of MEF2A, BNIP3, CDK5R1, HERC1, ITPR1, OPTN, UBQLN2, and USP33 were detected using qPCR. ANOVA followed by LSD test was used for comparison among groups. * p < 0.05 compared with the control group.

    Journal: Frontiers in Neuroscience

    Article Title: Decreased MEF2A Expression Regulated by Its Enhancer Methylation Inhibits Autophagy and May Play an Important Role in the Progression of Alzheimer’s Disease

    doi: 10.3389/fnins.2021.682247

    Figure Lengend Snippet: Expression levels of the screened seven genes after si-MEF2A transfection. (A–H) After BV2 cells transfected with three si-MEF2A for 48 h to knock down the expression of MEF2A, the expression levels of MEF2A, BNIP3, CDK5R1, HERC1, ITPR1, OPTN, UBQLN2, and USP33 were detected using qPCR. ANOVA followed by LSD test was used for comparison among groups. * p < 0.05 compared with the control group.

    Article Snippet: Total RNA was extracted using TRIzol ® Reagent (Life Technologies, Grand Island, NY, United States) and reverse transcribed into cDNA using PrimeScript TM II 1 st Strand cDNA Synthesis Kit for qPCR (TaKaRa, Tokyo, Japan) according to the manufacturer’s instructions.

    Techniques: Expressing, Transfection, Knockdown, Comparison, Control